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2 edition of characterization of mumps virus proteins. found in the catalog.

characterization of mumps virus proteins.

Edward John Barry Simpson

characterization of mumps virus proteins.

by Edward John Barry Simpson

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Published .
Written in English


Edition Notes

Thesis (Ph. D.)--The Queen"s University of Belfast, 1984.

The Physical Object
Pagination1 v
ID Numbers
Open LibraryOL19190013M

Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emergent virus responsible for a worldwide epidemic in The coronavirus spike proteins belong to class I fusion proteins, and are characterized by the existence of two heptad repeat (HR) regions, HR1 and HR2. The HR1 region in coronaviruses is predicted to be considerably longer than that in other type I virus fusion. CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): mRNA isolated from mumps virus-infected Vero cells was converted into cDNA and cloned into the PstI site of the plasmid pBR After screening with 32p-labelled cDNA synthesized from poly(A) + RNA of uninfected or mumps virus-infected Vero cells, five different groups of virus-specific clones were obtained.

CiteSeerX - Document Details (Isaac Councill, Lee Giles, Pradeep Teregowda): The SBL-1 strain of mumps virus, grouping with genotype A on the basis of the small hydrophobic protein gene sequence, was grown in the presence of three different monoclonal antibodies. The monoclonal antibodies were directed against the haemagglutinin–neuraminidase (HN) protein and they inhibited . Characterization ofVProtein in Measles Virus-Infected Cells encodeacysteine-rich protein designated V. the exact number depending on the virus strain (17). Six structural proteins have.

  These results are not dissimilar to those found in a study of Finnish children with “mumps-like illness,” only 17 (2%) of of whom had serological evidence of acute mumps virus infection. Another viral etiology was serologically detected in 84 (14%) of patients, including some viruses not detected by Hatchette and colleagues, such. The mumps virus (MuV) V protein was characterized in virus infected cells by the use of antipeptide sera. In radioimmune precipitation assay (RIPA), the sera reacted with the V protein and also immunoprecipitated the nucleocapsid (NP) and phospho (P) proteins. However, by depletion RIPA (in which either the NP and P proteins or the V protein were removed) and Western immunoblotting, it .


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Characterization of mumps virus proteins by Edward John Barry Simpson Download PDF EPUB FB2

clinical samples and to determine the genotype. Twelve genotypes of mumps virus are currently recognized by the World Health Organization, and the standard protocol for genotyping requires sequencing the entire gene coding for the small hydrophobic (SH) protein.

Phylogenetic analysis of the mumps samplesAuthor: Rebecca J. McNall, Adam K. Wharton, Raydel Anderson, Nakia Clemmons, Elena N. Lopareva, Carlos Gonza.

The crystal structure of the fusion protein core of Mumps virus (MuV) was determined at Å resolution. The complex is a six-helix bundle in which three HR1 peptides form a central highly hydrophobic coiled-coil and three HR2 peptides pack against the hydrophobic grooves on the surface of central coiled-coil in an oblique antiparallel by: 5.

The nucleocapsid protein (NP) of mumps virus (MuV), a paramyxovirus, was coexpressed with the phosphoprotein (P) in Escherichia coli. The NP and P proteins form a soluble complex containing RNA. Under a transmission electron microscope, the NP-RNA complex appears as a nucleocapsidlike ring that has a diameter of approximately 20 nm with 13 by:   Se- DETECTION AND CHARACTERIZATION OF MUMPS VIRUS V PROTEIN quencing 4 other cDNA clones showed that 2 clones (pMMP10 and pEMP5) contained 8 G residues while 2 others (pMGPl 5 and pEMP4) contained 6 at the region mentioned by: The fusion proteins of enveloped viruses mediating the fusion between the viral and cellular membranes comprise two discontinuous heptad repeat (HR) d Cited by: 5.

Detection and characterization of mumps virus V protein. Takeuchi K., Tanabayashi K., Hishiyama M., Yamada Y.K., Yamada A., Sugiura A. By cDNA cloning, DNA sequencing, and RNAse mapping analyses two distinct mRNA species were shown to be transcribed from the mumps virus P gene; one faithful and the other edited copies.

This, along with genomic characterization of the mumps virus, would provide the most complete picture of transmission. This is crucial and may aid in limiting the progression of active outbreaks. We applied this method on mumps virus strains collected from patients infected during the Arkansas outbreak.

Mumps is a common and highly contagious viral disease characterized by fever and swelling of the salivary glands. Although mumps infections are benign and usually not fatal, they rarely cause more serious neurological complications, such as aseptic meningitis, encephalitis, and deafness. Mumps virus.

Mumps was first described by Hippocrates in the fifth century BC, in his first Book of Epidemics, but a viral aetiology was not demonstrated until the s, when Johnson and Goodpasture fulfilled Koch’s postulates by transferring the disease from experimentally infected rhesus macaques (Macaca mulatta), to children in his neighbourhood, using a bacteria-free, filter-sterilized.

Mumps virus belongs to the family of Paramyxoviridae and has the potential to be an oncolytic agent. Mumps virus Urabe strain had been tested in the clinical setting as a treatment for human cancer four decades ago in Japan.

These clinical studies demonstrated that mumps virus could be a promising cancer therapeutic agent that showed significant antitumor activity against various types of cancers.

Mumps virus, like measles virus, is a paramyxovirus, containing a single-stranded negative-sense RNA genome. Before the advent of the mumps vaccine, mumps was the most common cause of viral meningitis, accounting for more than 15% of isolates.

Currently, mumps virus meningitis is rare in developed countries. Request PDF | Structural characterization of Mumps virus fusion protein core | The fusion proteins of enveloped viruses mediating the fusion between the viral and cellular membranes comprise two. Out of nine proteins coded by seven mumps genes, only four, HN, NP, M and P, were identified in mumps virus grown in CEF, whereas in mumps virus grown in Vero, additionally L and V proteins were detected (identification data are listed in Additional file 1: Table S1).

HN protein was detected in two and NP in three bands. Mumps virus (MuV) belongs to the family Paramyx. oviridae, genus Rubulavirus(11). The genome is a sin. gle stranded RNA with negative polarity, encoding seven.

proteins in the following order, 3'-nucleocapsid (N), phosphoprotein (P), matrix (M), fusion (F), small. hydrophobic (SH), haemagglutinin-neuraminidase (HN), and large protein (L)-5'(3). The SH gene of MuV is Mumps virus is a paramyxovirus in the same group as parainfluenza and Newcastle disease virus.

Parainfluenza and Newcastle disease viruses produce antibodies that cross-react with mumps virus. The virus has a single- stranded RNA genome. Structural characterization of mumps virus fusion protein core. The fusion proteins of enveloped viruses mediating the fusion between the viral and cellular membranes comprise two discontinuous heptad repeat (HR) domains located at the ectodomain of the enveloped glycoproteins.

The crystal structure of. Behnam Alirezaie, Khosrow Aghaiypour, Abbas Shafyi, Genetic characterization of RS‐12 (S‐12), an Iranian isolate of mumps virus, by sequence analysis and comparative genomics of F, SH, and HN genes, Journal of Medical Virology, /jmv, 80, 4, (), ().

The crystal structure of the fusion protein core of Mumps virus (MuV) was determined at A resolution. The complex is a six-helix bundle in which three HR1 peptides form a central highly hydrophobic coiled-coil and three HR2 peptides pack against the hydrophobic grooves on the surface of central coiled-coil in an oblique antiparallel manner.

Mumps virus is a member of the Paramyxoviridae family of enveloped, non-segmented, negative-sense RNA viruses. The signs of mumps include swelling of the parotid glands, salivary glands and other epithelial tissues. Symptoms of mumps are fatigue, body aches, headache, loss of appetite, low grade fever, swelling of the salivary glands.

The epidemiologic and genetic characteristics of mumps viruses detected in China from to were analyzed in this study. Mumps remains endemic in China with a high overall incidence rate.

The incidence of mumps in Western China was higher than that in other regions of the country. Each year. Request PDF | Characterization of Nucleocapsid Binding by the Measles Virus and Mumps Virus Phosphoproteins | We report an analysis of the interaction between the P protein .Mumps virus.

Mumps was first described by Hippocrates in the fifth century bc, in his first Book of Epidemics, but a viral aetiology was not demonstrated until the s, when Johnson and Goodpasture fulfilled Koch's postulates by transferring the disease from experimentally infected rhesus macaques (Macaca mulatta), to children in his.Plays an essential role in the inhibition of host immune response.

Prevents the establishment of cellular antiviral state by blocking interferon-alpha/beta (IFN-alpha/beta) production and signaling pathway.

Interacts with host IFIH1/MDA5 and DHX58/LGP2 to inhibit the transduction pathway involved in the activation of IFN-beta promoter, thus protecting the virus against cell antiviral state.